願大家 PCR 愉快     ：D

http://bio-rad.cnpg.com/lsca/videos/ScientistsForBetterPCR/

（Bio-rad 弄了個PCR之歌，也該為這改變生醫技術的里程碑致敬，只是…他們實在不該插入廣告的 ）

The PCR Song

歌詞

There was a time when to amplify DNA,
You had to grow tons and tons of tiny cells.
Then along came a guy named Dr. Kary Mullis,
Said you can amplify in vitro just as well.
Just mix your template with a buffer and some primers,
Nucleotides and polymerases, too.
Denaturing, annealing, and extending.
Well it’s amazing what heating and cooling and heating will do.
PCR, when you need to detect mutations.
PCR, when you need to recombine.
PCR, when you need to find out who the daddy is.
PCR, when you need to solve a crime.
(repeat chorus)

The Polymerase Chain Reaction

Introduction

1983年美國PE-Cetus公司人類遺傳研究室的Kary Mullis 等發明了具有劃時代意義的聚合酶連鎖反應 (Polymerase Chain Reaction; PCR)技術，獲1993年諾貝爾獎(Nobel Prize) 以來，PCR技術就被科學界公認：為近數十年來分子生物領域的最大成就之一。其原理類似於DNA的體內複製，只是在試管中給DNA的體外合成提供以致一種合適的條件---摸板DNA，寡核苷酸引子，DNA聚合酶，合適的緩衝體系，DNA變性、復性及延伸的溫度與時間。PCR技術能放大微量DNA產物，對於實驗室中已廣泛應用於遺傳疾病的診\斷、病原菌之偵測以及時下蠻熱門的CSI犯罪偵測上，未來應用無可限量。

Kary Mullis developed the technique of the polymerase chain reaction (PCR) in 1983. He won a Nobel Prize for the procedure in 1993 as it became quite clear PCR had revolutionized the worlds of biochemistry and genetics. PCR enables researchers to amplify extremely small samples of DNA, even one molecule, into virtually unlimited quantities. PCR is now a common laboratory procedure, and because of its broad range of applications, it is rapidly reaching the nonscientific community as well. For example, PCR is used in paternity testing. The genes of the father can be amplified and compared to the child’s DNA for similarities. Courts are also using PCR to compare genetic evidence found at a crime scene to that of a suspect’s DNA. This usage enables even the smallest amount of DNA to act as a witness to the crime allowing a greater resolution between guilt and innocence. PCR is also helping in the isolation of genes and the decoding of the human genome itself.